Lymphokine-activated Killer Cells in Rats: Analysis of Progenitor and Effector Cell Phenotype and Relationship to Natural Killer Cells1

The progenitor and effector cell phenotype of lymphokine-activated killer (LAK) cells generated in 1-344 rats by recombinant human interleukin 2 (IL-2) (rIL-2) were analyzed. Highly purified populations of peripheral blood large granular lymphocytes (LGL) exhaustively de pleted of I -cells were fully capable of generating high levels of LAK activity by 3 to 5 days in culture while purified populations of resting I cells devoid of LGL could not generate LAK activity. This pure population of LGL expressed surface markers characteristic of rat natural killer (NK) cells [i.e., OX8*, asialomonoganglioside (asialo-GM/), laminili4, OX19", R1-3B3", W3/25", la", surface immunoglobulin negative (SIg~)|. Further evidence that NK cells were the progenitors of cells with LAK activity was obtained by treatment of spleen or peripheral blood lymphocytes with anti-laminin or anti-asialo-GMi antibodies plus com plement or with the lysosomotropic agent L-leucine methyl ester. These treatments effectively depleted IX.I ./NK cell activity and the subsequent generation of rIL-2-induced LAK activity. Analysis of the LAK effector phenotype by cell sorting demonstrated that the majority of cells with LAK activity were OX8+, asialo-GMi% laminin*, OX6% OX19", Rl3B3~, W3/25", and SIg~. Furthermore, treatment of LAK cells with Lleucine methyl ester also significantly reduced their cytolytic activity. Thus, the LAK effector cells were also LGL and expressed surface marker characteristic of activated NK cells and not those of mature Tor B-cells. The proliferative response of rat spleen or blood lymphocytes to rlL2 appeared to be primarily associated with LGL/NK cells since depletion of NK cells by anti-asialo-GMi or anti-laminin antibody plus complement or by L-leucine methyl ester significantly (/' < 0.001) reduced the incorporation of [3H]thymidine into DNA. In contrast, depletion of I cells (by anti-T-cell antibody plus complement) did not significantly affect rIL-2-induced proliferation. Similarly, T-cell-depleted, highly purified populations of LGL gave substantial proliferative responses to rIL-2. These studies clearly indicate that in the rat, the major cell population activated by rIL-2 is the LGL/NK cell and these cells appear to represent the major population of cells in blood or spleen which generate broad antitumor (LAK) cytotoxicity.